The Leukocyte Common Antigen (LCA/T200), also known as CD45, is a membrane sialoglycoprotein that has three exons encoding peptide segments called A, B, and C, respectively. Differential splicing of your exons will produce various isoforms. CD45RA is considered a single-chain glycoprotein and is expressed in 40 to 50 percent of peripheral CD4+T cells. It is also expressed in 50 percent of peripheral CD8+ T-cells, leukemic B-cell lines, and B-cells.
More Information
This antibody is designed for research purposes. It does have a clone called SPM504. The immunogen is the stimulated human leucocytes, and it has an isotype of IgG2a. It has an undetermined epitope and a molecular weight of 205 to 220 kDa.
Applications/Procedures
The CD45RA antibody is designed to be used with Immunohistochemistry applications. You should only use paraffin-embedded or Formalin-fixed tissues with this antibody. Deparaffinized slides are required, and you can use xylene or one of its alternatives, as well as graded alcohols. If you’ve purchased the concentrated format, you will need to dilute the antibody using a ratio of one to 200. There is no method for antigen retrieval, though you will need to incubate it for 30 minutes at room temperature. You should wash the slides between steps with PBS/0.05% Tween.
The positive control for this antibody is the tonsil with cellular localization occurring in the membrane.
It is important that you do not freeze the antibody and keep it stored at a temperature of two to eight degrees Celsius. Likewise, you should not use the antibody if it is past its expiration date. Instead, you should purchase new antibodies for your research because your test results may be different.
The CD45RA can be used for many research needs. You can find everything you need at Spring Bioscience, so visit today.
